No.4（2009）3.Profiling of Reductively VOC Bacteria by Gene Analysis for Bioremediation

Kyoko Akiyama，Akira Monkawa

  It is well known that tetrachloroethylene (PCE) and trichloroethylene (TCE) are organic pollutants in soil and are used in various fields including the manufacture of semiconductors, metallic instrument processing, and in dry cleaning as cleaning agents or solvents. Bioremediation is an attractive method in the technological field of the cleanup of polluted environments based on natural processes. The detection of microbes by molecular biology is important to understand the effect of bioremediation and its influence on the ecosystem. Real-time PCR is a powerful tool for bioremediation to detect and enumerate the target bacteria that are directly related to the degradation of contaminants. The 16S ribosomal RNA (16S rRNA) gene and dehalogenase (RDase) genes provide fingerprinting of bacterial cells. We demonstrated that dechlorinated anaerobic bacteria such as Dehalococcoides sp. were detected by the Real-time PCR approach targeting 16S rRNA genes and three Dehalococcoides reductive dehalogenase (RDase) genes with assigned function (i.e., tceA, vcrA, and bvcA).

Keywords

VOC（Volatile Organic Compound），Bioremediation，Gene analysis，Real-time PCR.

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